Samples: immunoabsorption 26.11.18
- 18-858 HEK-absorbed (undiluted)
- 18-858 NF55-absorbed (undiluted)
- 18-858 1/50 (NF155 title: 1/24300)
- 18-610 1/50 (NF155 title: 1/2700)
- Ab anti-NF155 (15035, Cell Signal) 1/100
Protocol: NOVA Lite Monkey Cerebellum/Cerebrum and mouse stomach (504225-Inova)
- Samples diluted in PBS. 30 min at RT
- Wash 3 times with PBS.
- Secondary Ab FITC IgG Conjugate Primate Absorbed (undiluted). 30 min at RT
- In the case of ab anti-NF155 –> secondary ab GAR488 IgG 1/1000
- Wash 3 times with PBS.
- Mount with Fluoromount mounting medium.
Result:
- 1 and 2: no hay marcaje en las céls de Purkinje
- 3: se marcan claramente las céls de Purkinje
- 4: no hay marcaje en las céls de Purkinje
- 5: no hay marcaje –> anticuerpo muy diluido?
1: 18-858 HEK-absorbed (undiluted) 
2: 18-858 NF155-absorbed (undiluted) 
3: 18-858 1/50 
4: 18-610 1/50 
5: Ab anti-NF155 1/100
