20181127: IH Monkey Cerebellum (18-858 absorbed)

Samples:  immunoabsorption 26.11.18

1. 18-858 HEK-absorbed (undiluted)
2. 18-858 NF186-absorbed (undiluted)
3. 18-858 NF155-absorbed (undiluted)
4. LAC (1/100)
5. Negative control (1/100)

Protocol: NOVA Lite Monkey Cerebellum/Cerebrum and mouse stomach (504225-Inova)

• Samples diluted in PBS. 30 min at RT
• Wash 3 times with PBS.
• Secondary Ab FITC IgG Conjugate Primate Absorbed  (undiluted). 30 min at RT
• Wash 3 times with PBS.
• Mount with Fluoromount mounting medium.

Result: no se ven las células de Purkinje en ninguna de las muestras, aunque la immunoabsorción funcionó (está comprovado por HEK). Repetir poniendo otra muestra NF155+ con títulos más altos (para comprovar que la immuno se ha hecho correctamente).