Samples:
| 1. 18-859 | 7. 18-865 |
| 2. 18-860 | 8. Negative control |
| 3. 18-861 | 9. Positive control (18-851) |
| 4. 18-862 | 10. 18-858 NF155-absorbed (26.11.18) |
| 5. 18-863 | 11. 18-858 HEK-absorbed (26.11.18) |
| 6. 18-864 |
NF155 ICC:
- HEKs 293 Cells Transfection: 2018.09.04
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS: Sample 18-860 is positive for IgG towards NF155. The immunoabsorption worked well (the 18-858 NF155-absorbed is negative while 18-858 HEK-absorbed is positive).
| 1. 18-859 | – | 7. 18-865 | – |
| 2. 18-860 | + | 8. Negative control | – |
| 3. 18-861 | – | 9. Positive control (18-851) | + |
| 4. 18-862 | – | 10. 18-858 NF155-absorbed (26.11.18) | – |
| 5. 18-863 | – | 11. 18-858 HEK-absorbed (26.11.18) | + |
| 6. 18-864 | – |
