OBJECTIVE: To test IgM positivity towards NF155 in NF155 IgG CIDP patients.
SAMPLES: 18-695, 18-708, 18-774, 18-850, 18-858, LAC, 15-195, CTRL – A10A, CTRL – M35A.
NF155 ICC:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/40 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium
RESULTS: All samples were negative.
