Objective: to test a new GAH594 IgG antibody (109-585-003, Jackson)
CNTN1-CASPR1:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 with 5% goat serum in PBS.
- Negative control
- Positive control (18-808)
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000).
- Vectashield mounting medium
NF155:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 with 5% goat serum in PBS.
- Negative control
- Positive control (18-858)
- Primary antibody: AF3235 diluted 1/1000
- Secondary antibodies: GAC488 + GAH 594 IgG (1/1000).
- Vectashield mounting medium
RESULT: the new GAH594 antibody works better than the other antibodies that we were using.
