Objective:
- Detection of IgG positivity towards NF155/NF186/NF140 in CIDP patients.
- Identification of crossreactivity with non-transfected HEK cells in CMT patients
SAMPLES:
- For IgG: 23, 24, 18-854, 18-857; CTRL – 1, CTRL +
- For IgM: 43; 45; CTRL –
NF155, NF186 and NF140 ICC:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/40 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium
RESULTS: All samples wer negative; we are going to perform an IHC in rat ciatic nerve to confirm it.
