Objectives: To test CIDP patients for IgG positivity towards CNTN1/CASPR1.
Samples: 35-45, 47, 68-143
CNTN1/CASPR1 ICC:
- Transfection date: 2018.08.20
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium
RESULTS: 80 and 117 maybe are positive for CASPR1. We are going to perform an ICC only for CASPR1.
