Objective: Confirmation of IgG positivity towardd NF155, NF186, NF140 and CNTN1 of CIDP samples from Holland.
SAMPLES:
For NF155: 4, 17, 29, 83 115, 139
For NF140 and NF186: 29, 115, 139
For CNTN1: 99, 122
NF155, NF140, NF186 ICC:
- HEKs 293 Cells Transfection: 2018.08.14
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
RESULTS: All samples were positive except for 29.
