Objectives: To test CIDP patients for IgG positivity towards CNTN1.
Samples:
ID (samples from Holland) CNTN1 IgG Result
23 -
50 -
51 -
64 -
89 -
90 -
98 -
110 -
122 +
135 -
136 -
137 -
138 -
139 -
140 -
141 -
142 -
143 -
18-840 (ICQ) -
Positive control (15-198) +
Negative control -
Transfection: 14/8/18
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
Results:
- Sample 122 is positive for IgG towards CNTN1.
- The rest of the samples are negative
