Objectives: To test CIDP patients for IgG positivity towards NF140.
Samples: 68-74
| SAMPLE | NF 140 |
|---|---|
| 68 | - |
| 69 | - |
| 70 | - |
| 71 | - |
| 72 | - |
| 73 | - |
| 74 | - |
NF140 ICC:
- HEKs 293 Cells Transfection: 2018.07.26
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: All samples were negative
