Objectives: To test CMT patients for IgG positivity towards CNTN1.
Patients:
| NUMBER | NAME AND SURNAMES | CODE |
|---|---|---|
| 1 | Blai Batista | 056-16 |
| 2 | Gemma Carreras Colomer | 074-02 |
| 3 | Rosa Maria Martinez Fernandez | 191-01 |
| 4 | Asunción Nieto Romero | 107-01 |
| 5 | Enrique Torrats Fontanelles | 028-19 |
| 6 | Jose Torres Nieto | 107-02 |
| 7 | Ivan Patrici Santiago Varzulli | 177-16 |
| 8 | Pilar España Hervás | 166-04 |
| 9 | Prudencio Colmenero Lázaro | |
| 10 | Luis Piqué Vilanova | |
| 11 | M. Luz Cote Sánchez | 211-16 |
| 12 | Joan Navarro | 212-03 |
| 13 | Gemma Hurtado Osorio | 212-04 |
| 14 | R. Rodriguez Cote | 212-10 |
| 15 | Teresa Gené Escoda | 212-13 |
| 16 | Irene Arimany | 213-08 |
| 17 | Fina Alexandra | 213-12 |
| 18 | Jaume Crusellas | 213-13 |
| 19 | Joan Puigdover | 213-14 |
| 20 | Ramon Gomera (PnP DM) | 213-11 |
| 21 | Gerardo Martínez Martínez | |
| 22 | Pere Cussó | |
| 23 | CTRL- |
CNTN1 ICC:
- HEKs 293 Cells Transfection: 20.02.2018
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
Results: All samples were negative.
