Objectives: To test sera 18-594 for IgG positivity towards CASPR1.
Patients: 18-594, CTRL- and CTRL+ (15-198).
CASPR1 and Doble ICC:
- HEKs 293 Cells Transfection: 14.09.2016 for CASPR1 and 16.01.2018 for Doble.
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: 133634 diluted 1/250.
- Secondary antibodies: GAR 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: All samples were negative.
