Objectives: To test CIDP and CMT patients for IgM positivity towards CNTN1.
Patients:
CIDP patients:
| NUMBER | CODE |
|---|---|
| 1 | 18-549 |
| 2 | 18-550 |
| 3 | 18-551 |
| 4 | 18-552 |
| 5 | 18-553 |
| 6 | 18-554 |
| 7 | 18-555 |
| 8 | 18-556 |
| 9 | 18-557 |
| 10 | 18-558 |
| 11 | 18-559 |
| 12 | 18-560 |
| 13 | 18-561 |
| 14 | 18-562 |
| 15 | 18-563 |
| 16 | 18-564 |
| 17 | 18-565 |
| 18 | 18-566 |
| 19 | 18-567 |
| 20 | 18-568 |
| 21 | 18-569 |
| 22 | 18-570 |
| 23 | 18-571 |
| 24 | 18-572 |
| 25 | 18-573 |
| 26 | 18-575 |
| 27 | 18-576 |
| 28 | 18-577 |
| 29 | 18-578 |
| 30 | 18-579 |
| 31 | 18-580 |
CMT patients:
| NUMBER | NAME AND SURNAMES | CODE |
|---|---|---|
| 1 | Blai Batista | 056-16 |
| 2 | Gemma Carreras Colomer | 074-02 |
| 3 | Rosa Maria Martinez Fernandez | 191-01 |
| 4 | Asunción Nieto Romero | 107-01 |
| 5 | Enrique Torrats Fontanelles | 028-19 |
| 6 | Jose Torres Nieto | 107-02 |
| 7 | Ivan Patrici Santiago Varzulli | 177-16 |
| 8 | Pilar España Hervás | 166-04 |
| 9 | Prudencio Colmenero Lázaro | |
| 10 | Luis Piqué Vilanova | |
| 11 | M. Luz Cote Sánchez | 211-16 |
| 12 | Joan Navarro | 212-03 |
| 13 | Gemma Hurtado Osorio | 212-04 |
| 14 | R. Rodriguez Cote | 212-10 |
| 15 | Teresa Gené Escoda | 212-13 |
| 16 | Irene Arimany | 213-08 |
| 17 | Fina Alexandra | 213-12 |
| 18 | Jaume Crusellas | 213-13 |
| 19 | Joan Puigdover | 213-14 |
| 20 | Ramon Gomera (PnP DM) | 213-11 |
| 21 | CTRL- |
CNTN1 ICC:
- HEKs 293 Cells Transfection: 15.02.2018
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/10 for IgM with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
Results: All samples were negative.
*We run out of serum 18-574 and we change serum 18-578 for serum of the same patient in seroteca 2009: 09-2464 (Box 76).
