Objectives: To test CMT patients for IgM and IgG positivity towards NF140, NF186 and NF155.
Patients:
| NUMBER | NAME AND SURNAMES (DIAGNOSIS) | CODE | RESULTS IgG | RESULTS IgM |
|---|---|---|---|---|
| 1 | M. Luz Cote Sánchez (CMT1A) | 211-16 | - | + (for NF140 and NF186) |
| 2 | Joan Navarro (CMT) | 212-03 | - | - |
| 3 | Gabriele Hurtado Osorio (CMT) | 212-04 | - | - |
| 4 | Raquel Rodriguez Cote (CMT) | 212-10 | - | - |
| 5 | Teresa Gene Escora (CMT) | 212-13 | - | ? (for NF140 and NF186) |
| 6 | Irene Arimany Sánchez (CMT) | 213-08 | - | ? (for NF140 and NF186) |
| 7 | Fina Alejandra (CMT4G) | 213-12 | - | - |
| 8 | Jaume Crusellas (CMT4) | 213-13 | - | - |
| 9 | Joan Puigdolvers (CMT1A) | 213-14 | - | - |
| 10 | Ramon Gomera (PnP DM) | 213-11 | - | - |
| 11 | CTRL - | - | - | |
| 12 | CTRL + | - | - |
NF140 ICC:
- HEKs 293 Cells Transfection: ?
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM and diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies:
- For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- For IgM: GAC 488 (1/1000) + GAH 594 IgM (1/1000)
- Vectashield mounting medium.
NF186 ICC:
- HEKs 293 Cells Transfection: Different dates of transfection
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM and diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies:
- For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- For IgM: GAC 488 (1/1000) + GAH 594 IgM (1/1000)
- Vectashield mounting medium.
NF155 ICC:
- HEKs 293 Cells Transfection: 2018.01.16
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM and diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies:
- For IgG: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- For IgM: GAC 488 (1/1000) + GAH 594 IgM (1/1000)
- Vectashield mounting medium.
Results: The sample 211.16 (CMT1A) was clearly positive for IgM towards NF140 and NF186, and the samples 213-08 and 213-12 were mild positive for IgM towards NF140 and NF186. All samples were negative for IgG towards NF140, NF186 and NF155.
