Objectives: To test CIDP patients for IgM positivity towards NF140.
Patients:
| NUMBER | CODE |
|---|---|
| 1 | 18-502 |
| 2 | 18-503 |
| 3 | 18-504 |
| 4 | 18-505 |
| 5 | 18-506 |
| 6 | 18-507 |
| 7 | 18-508 |
| 8 | 18-555 |
| 9 | 18-556 |
| 10 | 18-557 |
| 11 | 18-558 |
| 12 | 18-559 |
| 13 | 18-560 |
| 14 | 18-561 |
| 15 | 18-562 |
| 16 | 18-563 |
| 17 | 18-564 |
| 18 | 18-565 |
| 19 | 18-566 |
| 20 | 18-567 |
| 21 | 18-568 |
| 22 | 18-569 |
| 23 | 18-570 |
| 24 | 18-571 |
| 25 | 18-572 |
| 26 | 18-573 |
| 27 | 18-574 |
| 28 | 18-575 |
| 29 | 18-576 |
| 30 | 18-577 |
| 31 | 18-578 |
| 32 | 18-579 |
| 33 | 18-580 |
| 34 | CTRL - |
NF140 ICC:
- HEKs 293 Cells Transfection: 2018.01.16
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAH 594 IgM (1/1000) + GAC 488 (1/1000).
- Vectashield mounting médium.
Results: All samples were negative.
