Objectives: To test CMT patients for IgG and IgM positivity towards NF155.
Patients:
| Number | Name and Surnames | Code |
|---|---|---|
| 1 | Blai Batista Roige | 056-16 |
| 2 | Gemma Carreras Colomer | 074-02 |
| 3 | Rosa Maria Martinez Fernandez | 191-01 |
| 4 | Asunción Nieto Romero | 107-01 |
| 5 | Enrique Torrats Fontanelles | 028-19 |
| 6 | Jose Torres Nieto | 107-02 |
| 7 | Ivan Patrici Santiago Varzulli | 177-16 |
| 8 | Pilar España Hervás | 166-04 |
| 9 | CTRL - Edu | |
| 10 | CTRL + 15-195 | 15-195 |
NF155 ICC:
- HEKs 293 Cells Transfection: 2017.11.22
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG and 1/10 for IgM with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies:
- For IgG: GAH 594 IgG (1/1000) + GAC 488 IgG (1/1000).
- For IgM: GAH 594 IgM (1/1000) + GAC 488 IgG (1/1000).
- Vectashield mounting medium
Results: All samples were negative.
