Objectives: To test CIDP patients for IgM positivity towards NF140.
Patients:
Number | Code |
---|---|
1 | 18-509 |
2 | 18-510 |
3 | 18-511 |
4 | 18-512 |
5 | 18-513 |
6 | 18-514 |
7 | 18-515 |
8 | 18-516 |
9 | 18-517 |
10 | 18-518 |
11 | 18-519 |
12 | 18-520 |
13 | 18-521 |
14 | 18-522 |
15 | 18-523 |
16 | 18-524 |
17 | 18-525 |
18 | 18-526 |
19 | 18-527 |
20 | 18-528 |
21 | 18-529 |
22 | 18-530 |
23 | 18-531 |
24 | CTRL - Edu |
NF140 ICC:
- HEKs 293 Cells Transfection: 2017.08.30
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/10 for IgM with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAH 594 IgM (1/1000) + GAC 488 IgG (1/1000).
- Vectashield mounting medium
Results: All samples were negative.