Objectives
To test Zika-GBS patients for IgG positivity towards human NrCAM in HEK 293 transfected cells.
Patients
| codio laboratorio | COD | GENDER | AGE | DX-COD |
|---|---|---|---|---|
| 1 | ZN001A | 1 | 44 | GUILLAIN BARRÉ SYNDROME |
| 27 | ZN002A | 0 | 53 | GUILLAIN BARRÉ SYNDROME |
| 5 | ZN004A | 0 | 47 | GUILLAIN BARRÉ SYNDROME |
| 57 | ZN006A | 0 | 38 | GUILLAIN BARRÉ SYNDROME |
| 21 | ZN007A | 0 | 43 | GUILLAIN BARRÉ SYNDROME |
| 18 | ZN008A | 0 | 47 | GUILLAIN BARRÉ SYNDROME |
| 15 | ZN010A | 0 | 27 | GUILLAIN BARRÉ SYNDROME |
| 4 | ZN011A | 1 | 42 | GUILLAIN BARRÉ SYNDROME |
| 33 | ZN012A | 1 | 22 | Encephalitis |
| 9 | ZN013A | 1 | 49 | GUILLAIN BARRÉ SYNDROME |
| 2 | ZN014A | 0 | 70 | GUILLAIN BARRÉ SYNDROME |
| 44 | ZN015A | 0 | 34 | GUILLAIN BARRÉ SYNDROME / BICKERSTAFF |
| 40 | ZN016A | 0 | 41 | GUILLAIN BARRÉ SYNDROME |
| 46 | ZN017A | 1 | 58 | GUILLAIN BARRÉ SYNDROME |
| 13 | ZN018A | 1 | 38 | GUILLAIN BARRÉ SYNDROME |
| 19 | ZN019A | 1 | 10 | GUILLAIN BARRÉ SYNDROME |
| 30 | ZN020A | 1 | 26 | Peripheral facial palsy |
| 10 | ZN022A | 1 | 69 | GUILLAIN BARRÉ SYNDROME |
| 25 | ZN023A | 0 | 33 | Encephalitis |
| 48 | ZN025A | 1 | 8 | Transverse myelitis |
| 47 | ZN026A | 1 | 62 | GUILLAIN BARRÉ SYNDROME |
| 12 | ZN027A | 0 | 22 | Transverse myelitis |
| 51 | ZN039A | 0 | 18 | GUILLAIN BARRÉ SYNDROME |
| 36 | ZN040A | 0 | 41 | Transverse myelitis |
| 17 | ZN041A | 1 | 45 | GUILLAIN BARRÉ SYNDROME |
| 41 | ZN043A | 1 | 42 | GUILLAIN BARRÉ SYNDROME |
| 28 | ZN044A | 0 | 25 | GUILLAIN BARRÉ SYNDROME |
| 59 | ZN045A | 1 | 23 | Peripheral facial palsy |
| 34 | ZN046A | 1 | 49 | GUILLAIN BARRÉ SYNDROME |
| 20 | ZN047A | 1 | 25 | Myeloradiculopathy |
| 45 | ZN048A | 0 | 27 | GUILLAIN BARRÉ SYNDROME |
| 22 | ZN052A | 0 | 27 | GUILLAIN BARRÉ SYNDROME |
| 58 | ZN055A | 1 | 54 | GUILLAIN BARRÉ SYNDROME |
| 23 | ZN056A | 0 | 23 | Transverse myelitis |
| 43 | ZN057A | 0 | 42 | GUILLAIN BARRÉ SYNDROME |
| 32 | ZN058A | 1 | 40 | Peripheral facial palsy |
Las muestras LAB ID 6,11, 26, 56, 55 y 54 no se han podido analizar por falta de suero.
NrCAM ICC
- Human NrCAM transfected HEKs 293 grown in coverslips (plates). Fixed with PFA 4%, permeabilized and blocked with 5% goat serum in PBS.
- PBS
- Blocking solution: 5% goat serum in PBS
- Patient’s sera diluted 1/100 (IgG) with 5% goat serum in PBS.
- Primary Ab: Rat anti-HA 1/2000
- Secondary Abs: GARat 488 (1/500)+ GAH 594 IgG (1/500)
- Vectashield mounting medium
Results
All samples are negative.
Samples 47 & 34 (although negatives) have a strong membrane staining (possible candidates for 2D experiments)
Sample 2 (although negative) has a characteristic dot-like staining pattern.
