Objectives
To test paraneoplastic neuropathy patients (Clínic) for IgG positivity towards human L1CAM in HEK 293 transfected cells.
Patients
- A1) 95-43
- A2)94-292
- A3) 97-543
- A4) 93-40
- A5) 92-147
- A6) 94-55
- B1) 93-38
- B2) 92-133
- B3) 01-848
- B4) 00-1289
- B5) 98-14
- B6) 03-1167
- C1) 04-2
- C2) 13-1127
- C3) 15-2420
- C4) 15-1131
- C5) 11-1167
- C6) 00-307
- D1) 15-2373
- D2) 08-530
- D3) 00-1248
- D4) 90-92
- D5) 04-726
- D6) 92-38
- E1) 98-164
- E2) 03-838
- E3) 05-409
- E4) 04-1323
- E5) 16-3512
- E6) 01-203
- F1) 93-94
- F2) 97-506
- F3) 02-616
- IRB 15-467
- AMC 15-107
L1CAM ICC
- Human L1CAM transfected HEKs 293 grown in coverslips (plates). Fixed with PFA 4%, permeabilized and blocked with 5% goat serum in PBS.
- PBS
- Blocking: 5% goat serum in PBS
- Patient’s sera diluted 1/100 (IgG) with 5% goat serum in PBS.
- Primary Ab: Mouse-anti-Myc (CBL430 Millipore) 1/200
- Secondary Abs: GAM 488 (1/500)+ GAH 594 (1/500) IgG
- Vectashield mounting medium
Results
All samples are negative.