Objectives
To test paraneoplastic neuropathies patients for IgG positivity towards human Piezo 1 in HEK 293 transfected cells.
Patients
- B5) 98-14
- B6) 03-1167
- C1) 04-2
- C2) 13-1127
- C3) 15-2420
- C4) 15-1131
- C5) 11-1167
- C6) 00-307
- D1) 15-2373
- D2) 08-530
- D3) 00-1248
- D4) 90-92
- D5) 04-726
- D6) 92-38
- E1) 98-164
- E2) 03-838
- E3) 05-409
- E4) 04-1323
- E5) 16-3512
- E6) 01-203
- F1) 93-94
- F2) 97-506
- F3) 02-616
- IRB (15-467)
PIEZO 1 ICC
- Human Piezo 1 transfected HEKs 293 grown in coverslips . Fixed with PFA 4% and blocked with 5% goat serum in PBS. NOTE: Patient 00-1248 (IP) was also tested in Piezo 1 transfected and permeabilized HEK cells.
- PBS
- Blocking: 5% goat serum in PBS
- Patient’s sera diluted 1/100 (IgG) with 5% goat serum in PBS.
- Primary Ab: No primary antibody was used as cells incorporated GFP
- Secondary Abs: GAH 594 (1/500) IgG
- Vectashield mounting medium
RESULTS
All samples are negative.
