OBJECTIVE The same as previous ones. In this case changing the CD3 color (BD, CD3-PE_Cy7 clone SK7) MATERIALS Same as previous experiments, except the CD3.…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…