Samples: 239-265, 269, 274-275, 277-279, 281, 283, 288-295, 297-298, CTRL – 1, CTRL + (153). ANO2 ICC: HEKs 293 Cells Transfection: 11.12.18 Blocking solution: Goat…
11.12.18: Starter Amb una punta es rasca una mica del glicerolat sense descongelar-lo Posar-la a un tub falcon de bacteris amb 5 mL de LB…
Samples: from MS database 153 153 (without primary antibody) 251 101 102 Negative control ANO2 ICC: HEKs 293 Cells Transfection: 04.09.18 Blocking solution: Goat Serum 5…
Samples: 153 PROTOCOL: Fixation: in acetone 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker(DakoPen) to highlight the…
Objectives: To test an MS patient for IgG positivity towards ANO2 and check the crossreacrtivity between antibodies. Samples: 251 , CTRL + (217) and CTRL…
Objectives: To test an MS patient for IgG positivity towards ANO2 and check the crossreacrtivity between antibodies. Samples: 251 , CTRL + (153) and CTRL…
Objectives: To test MS patients for IgG positivity towards ANO2 Samples: ANO2 ICC: HEKs 293 Cells Transfection: 14.05.2018 Blocking solution: Donkey Serum diluted 3% in…
Objectives: To test if the change of the blocking medium and the secondary antibodies, are better for the ANO2 test. Patients: ANO2 ICC: HEKs…
Day 1- 2018.05.08: Prepare 5 plates of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for 1h…
Objetives: Check the cross-reactivity of the secondary antibodies Check if the permeabilization is better or not for the ICC Patients: ANO2 ICC: HEKs 293…