OBJECTIVES – To set up a protocol to analyze the CD19+ IL10+ subset in PBMCs of a control sample (Luis). – To determine whether the…
OBJECTIVE To analyze if B 10 cells after stimulation produce higher amount of IL 10 MATERIALS PBMCs (frozen cells) Antibodies: CD19-FITC, IL10 APC, Isotype controls (FITC and APC)…
OBJECTIVE To evaluate the amount of B cells producing IL10 To analyze if they all are plasma cells as previously reported To compare 2 different…
OBJECTIVE Set up Flow cytometry protocol to characterize B cell population and IL10. PROCEDURE 1. Isolate PBMCs from whole blood with Ficoll-Paque. – Dilute blood…
OBJECTIVE Set up Flow cytometry protocol to characterize B cell population and IL10. PROCEDURE 1. Thawing cells. – Wash 2 times frozen cells to remove…
OBJECTIVE Set up Flow cytometry protocol to characterize B cell population. PROCEDURE 1. Isolate PBMCs from whole blood with Ficoll-Paque. – Dilute blood sample 1:1…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against CNTN1/CASPR1 complex in these subsets. MATERIALS…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against NF155 in these subsets. MATERIALS Same…
OBJECTIVE To set-up a sorting protocol to isolate the B-cell subset responsible of producing pathogenic antibodies in neuropathies. The whole process is copied/adapted from Chihara…
OBJECTIVE The same as previous ones. In this case changing the CD3 color (BD, CD3-PE_Cy7 clone SK7) MATERIALS Same as previous experiments, except the CD3.…