OBJECTIVE Test patients that needed to be repeated from last experiment and check reactivity over Schwann cells fixed with PFA of those patients testing positive…
OBJECTIVE Test patients and controls after setting up the assay PROCEDURE We only perform live staining because it seems to be more sensitive according to…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against CNTN1/CASPR1 complex in these subsets. MATERIALS…
OBJECTIVE To sort plasmablasts, naive B cells and memory B cells to see if we can detect antibodies against NF155 in these subsets. MATERIALS Same…
OBJECTIVE To set-up a sorting protocol to isolate the B-cell subset responsible of producing pathogenic antibodies in neuropathies. The whole process is copied/adapted from Chihara…
OBJECTIVE Set up Schwann cell ICC to test the presence of antibodies against Schwann cells in CIDP patients MATERIALS Schwann cell line (P3), Innoprot PFA…
OBJECTIVE The same as previous ones. In this case changing the CD3 color (BD, CD3-PE_Cy7 clone SK7) MATERIALS Same as previous experiments, except the CD3.…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…
OBJECTIVE Set up FACS rotocol to characterize B cell subpopulations. PROCEDURE Isolate PBMCs from healthy donor with Ficoll-Paque (followed Ficoll protocol) Washed 2x with PBS…