Objective Amplify DNA to transfect HEK (CNTN-GFP and NF155) Materials One Shoot TOP10 Chemically competent E. coli transfected with NF155 (pCMV6-NF155-DDKMyc (Human)) One Shoot TOP10…
Objective In the last ICC we can not see positive marks for antibody against contactin, so we decide to test the new aliquote, the old…
Objective To test 3 new patients sera (Katia Adsvar, JoseM Gutierrez, Pablito Macatangay). Materials NF155-transfected HEK cells, grown in coverslips and freezed CNTN1-transfected HEK cells,…
Objective To test a new patient serum Materials NF155-transfected HEK cells, grown in coverslips and freezed CNTN1-transfected HEK cells, grown in coverslips and freezed Sera…
Objective To test a new patient Roberto Alonso Iglesias Materials NF155-transfected HEK cells, grown in coverslips and freezed CNTN1-transfected HEK cells, grown in coverslips and…
Objective To test a new patient Materials NF155-transfected HEK cells, grown in coverslips the day before CNTN1-transfected HEK cells, grown in coverslips the day before…
OBJECTIVE To set up a contactin-1 ELISA to titrate antiCNTN1+ patients’ sera. MATERIALS Nunc MaxiSorp ELISA plates HVH1 patient sera Recombinant Human CNTN1 protein (Sino…
OBJECTIVE To test reactivity against DRG neurons in patients with CIDP MATERIALS DRG neurons plated on coverslips the on 12.06.2013. We followed Liu et al…
OBJECTIVE Test patients that needed to be repeated from last experiment and check reactivity over Schwann cells fixed with PFA of those patients testing positive…
OBJECTIVE Test patients and controls after setting up the assay PROCEDURE We only perform live staining because it seems to be more sensitive according to…